GWYRE

GWYRE 2.0

The GWYRE resource contains modeled and experimentally determined structures of human proteins and protein complexes, annotated by phenotypic effects of genetic mutations. Future releases will contain proteins and protein complexes from other organisms, extended search options, and advanced scoring of residue mutations.

The project is a collaboration of Vakser Lab, The University of Kansas and Sternberg Lab, Imperial College London

Download all complexes

Database Status

The project offers a comprehensive resource of quaternary structures (experimental and/or modeled by AI-based AlphaFold) of experimentally verified protein-protein interactions (derived from the IntAct and BioGRID databases). It also includes an assessment of the phenotypic effects of genetic mutations with a special focus on amino acids substitutions on and in the nearest vicinity of protein-protein interfaces. Both chains of all binary complexes, currently processed in GWYRE, are either extracted from the corresponding PDB file (denoted as experimental on the Search and Visualization page) or modeled as one entity by AlphaFold version 2.3 (Alphafold2 fold-and-dock model).

Search and Download

Find all binary complexes with specified protein/gene

Search tool offers finding all binary complexes associated with specific protein/gene by entering whole/part of protein/gene ID(s) or name(s). Search results can be visualized by each pair of the chains in the search results with the opportunity to explore multiple interfaces for the same protein pair as well as variant information mapped on the specific interface (if available). Download tool offers a structure file in the cif (for experimental structures) of PDB format (model structures) along with a text file with the relevant information.